Forest Floor Mass, Organic Matter and Chemistry (Ca, Mg, K, P, Mn and some trace metals): Watershed 1 in 2000 (after the calcium addition)
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TITLE
Forest Floor Mass, Organic Matter and Chemistry (Ca, Mg, K, P, Mn and some trace metals): Watershed 1 in 2000 (after the calcium addition)
PRINCIPAL INVESTIGATOR(s)
Charley Driscoll
Department of Civil and Environmental Engineering, Syracuse University
Syracuse, NY
USA
Scott Bailey
USDA Forest Service, Hubbard Brook Experimental Forest
Campton, NH 03223
USA
Joel Blum
Department of Geological Sciences, The University of Michigan
Ann Arbor, MI 48109
USA
Don Buso
Institute of Ecosystem Studies, Hubbard Brook Experimental Forest
Campton, NH 03223
USA
Chris Eagar
USDA Forest Service, Northeastern Research Station
Durham, NH 03824
USA
Tim Fahey
Department of Natural Resources, Cornell University
Ithaca, NY 14853
USA
Melany Fisk
Department of Natural Resources, Cornell University
Ithaca, NY 14853
USA
Peter Groffman
Institute of Ecosystem Studies
Millbrook, NY 12545
USA
Chris Johnson
Department of Civil and Environmental Engineering, Syracuse University
Syracuse, NY 13244
USA
Gene Likens
Institute of Ecosystem Studies
Millbrook, NY 12545
USA
Thomas G. Siccama
Yale School of Forestry and Environmental Studies
New Haven, CT 06511
USA
OTHERS INVOLVED
Thomas G. Siccama
Yale School of Forestry and Environmental Studies
New Haven, CT 06511
USA
Chris Johnson
Department of Civil and Environmental Engineering, Syracuse University
Syracuse, NY 13244
USA
Tim Fahey
Department of Natural Resources, Cornell University
Ithaca, NY 14853
USA
Steve Hamburg
Center for Environmental Studies, Urban Ecology Laboratory, Brown University
Providence, RI 02912
USA
BEGIN DATE
1996
END DATE
Ongoing
LOCATION
Watershed 1 is a 11.8 ha watershed extending from the weir at 488 m to the ridge at 747 m
West bounding coordinate: -71.731339
East bounding coordinate: -71.726311
North bounding coordinate: 43.959286
South bounding coordinate: 43.952053
Elevation
Minimum: 488
Maximum: 747
(Unit: meter)
SAMPLING BACKGROUND
Hydrology has been monitored since 1956, and stream water chemistry has been monitored since 1963. The watershed is forested by typical northern hardwood species (sugar maple, beech and yellow birch) on the lower 90 % of its area, and by a montane boreal transition forest of red spruce, balsam fir and white birch on the highest 10%. Surveying techniques were used in 1996 to install a grid system of 200 grid cells, each 25 x 25 m square (slope corrected). These grid units serve as the coordinate system for sample plot selection. For more spatial information please visit the following links: http://www.hubbardbrook.org/watersheds/maps/w1_grid_map.htm and http://www.hubbardbrook.org/watersheds/w1_grid_elevations.htm.
The watershed was surveyed in 1996 and calcium was added in October 1999. A pre-calcium addition forest floor survey was completed in 1996, and another in 1998. The first post-treatment survey was conducted in 2000. This data set was collected in the summer of 2000. Repeated sampling at 1 to 5 year intervals is anticipated to continue.
SAMPLING DESIGN
Each row of the Watershed 1 grid system of 25 x 25 m cells was considered a stratified sampling set and one or more cells was randomly chosen from these lines as cells to be sampled. Some rows are short, with only 1 to 4 cells, and others are longer, up to 9 cells across. The longer rows may have 3 or 4 cells sampled. The short rows may not have any cells sampled. The location of the sampling plot within each cell was selected by an x-y random number system and the location determined by pacing in to the selected coordinates from the corner of the 25 x 25 m cell. 100 of the 200 cells were sampled in 2000.
FIELD COLLECTION PROCEDURES
Once a sampling point was located by pacing, a 15 x 15 cm wooden template was placed on the forest floor and 4 nails were placed through pre-drilled holes, one on each corner. This served to hold the template in place on the forest floor and retain the forest floor material within the sample area beneath the template. A keyhole saw, clippers and a trowel were used to make a clean cut around the template, thus isolating a block of forest floor. The surrounding forest floor material was scraped back from the block leaving it as a pedestal. This block was then undercut in the mineral soil with a saw or trowel to sever any roots. Using a trowel slid beneath the block and our hands on top, the block was carefully picked up and turned over such as to rest on the wooden template. Any remaining mineral soil was carefully scraped away "up" to the bottom of the Oa layer of the forest floor. After all such mineral soil was removed, the total thickness of the block was measured in two places on each of the 4 sides (8 measurements). The thickness of only the Oie was measured in the same manner. Oa thickness was later determined by subtraction of the average Oie thickness from the average total thickness for each block. The block was then separated into Oie and Oa, and each placed in a separate plastic bag for return to the laboratory.
NOTES
Long term forest floor sampling has been carried out on Watershed 6 since 1970. Watershed 1 was added to this sampling in 1996 as part of the calcium addition experiment.
LABORATORY PROCEDURES
The Oie was oven dried at 80 oC to a constant weight, and ground in a Wiley mill to pass a 2 mm screen. Large solid sticks and roots larger than the size of a pencil and obviously alive or fresh, were removed and weighed. These were not ground and were not included in the Oie forest floor mass calculations. The Oa was air dried, weighed and then sieved through a 0.5 cm stainless steel screen. Sticks, roots and debris (coarse fraction) not passing through the screen with mild pressure were weighed. Stones which were rarely present in the Oa, were also included in the coarse fraction. Material passing through the screen was weighed and rebagged. A subsample of this air dry screened soil was weighed, oven dried at 80 oC and weighed again. Then an air dry to oven dry conversion factor was determined in order to express the total Oa soil mass on an oven dry weight basis. The oven dried subsample of Oa was ground in a Wiley mill to pass a 2 mm screen and stored in a paper envelope. Samples of the ground/screened Oie and Oa were put in 4 oz. glass jars for long term preservation in the Hubbard Brook archives. All residual material, including sticks and roots screened out of the samples, have been put in boxes for preservation.
For determination of organic content and chemical analysis, a 0.5 g sample of the oven dry ground material was placed in a pre-weighed crucible and ashed overnight at 500 oC. The ashed material was cooled and weighed. The ash was eluted with 8 ml of 6N HNO3 and heated for a few minutes (to a simmer) on a hot plate. The hot extract solution was poured into a filter paper (Whatmann # 41 ashless) in a funnel and into a 50 ml volumetric flask. The crucible and the material on the filter paper were rinsed several times with distilled deionized water. Following these several rinsings, the volume in the flask was brought up to 50 ml with distilled deionized water. The 50 ml of extract solution was then placed in 60 ml plastic bottles for storage until chemical analysis. The ash remaining on the filter paper was saved and later put in 4 oz. jars. The elements Ca, Mg, K, P, Mn, Zn, Pb and Cu were determined for each sample using an ICP spectrometer. The ash remaining on the filter paper will be dissolved in hydrofluoric acid and analyzed for these elements. Carbon and nitrogen were determined using oven dried ground material on a CHN analyzer--C and N data are not yet available. These elements are being analyzed by Steve Hamburg at Brown University.
CALCULATIONS
Concentrations of elements in the forest floor were calculated on an oven dry weight basis. Total amounts of the elements were estimated on a per square meter basis by extrapolating up from the 15 x 15 cm sample mass. Forest floor thickness, species contributing litter to the sample site, and E horizon intensity for each plot are given in dataTable 1. Total mass, organic matter mass, and LOI of the forest floor are presented in dataTable 2. Amounts of Ca, Mg, K, P, Mn, Cu, Zn and Pb are presented in dataTable 3. Concentrations of Ca, Mg, K, P, Mn, Cu, Zn and Pb are presented in dataTable 4.
DATA ACCESS GUIDELINES
Publications using these data from the Hubbard Brook Ecosystem Study must include the following paragraph: Some data used in this publication were obtained by scientists of the Hubbard Brook Ecosystem Study; this publication has not been reviewed by those scientists. The Hubbard Brook Experimental Forest is operated and maintained by the Northeastern Research Station, U.S. Department of Agriculture, Newtown Square, Pennsylvania. Please note that data sharing and availability policy varies among the datasets available. Publishers of Hubbard Brook data are encouraged to contact the original data provider to obtain information for an acknowledgement of the original funding source of the research.
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NOTES ON DATA
Original field data sheets are in Greeley Lab, 370 Prospect Street, Yale School of Forestry and Environmental Studies. Samples of forest floor material in jars will be located in the USFS archive building at the HBEF in Woodstock, NH. Residual material (extra) is in boxes at Greeley Lab at the Yale Forestry School.
CONTACT PERSON
Thomas G. Siccama
Yale School of Forestry and Environmental Studies
New Haven, CT 06511
USA

Phone: (203)-432-5140
Email: tgs3@pantheon.yale.edu

Data file: w100ffa.txt
Description: Hubbard Brook Forest Floor Data - Watershed 1 2000 - 100 15x15 cm excavated blocks Element amounts
Column Variable Description Units Coded? Missing value label
1 Plot Plot# none y none
2 Calcium (g/m2) Oie Calcium amount for Oie (g/m2) gramsPerSquareMeter n none
3 Calcium (g/m2) Oa Calcium amount for Oa (g/m2) gramsPerSquareMeter n none
4 Calcium (g/m2) Total Calcium amount for forest floor in total (g/m2) gramsPerSquareMeter n none
5 Magnesium (g/m2) Oie Magnesium amount for Oie (g/m2) gramsPerSquareMeter n none
6 Magnesium (g/m2) Oa Magnesium amount for Oa (g/m2) gramsPerSquareMeter n none
7 Magnesium (g/m2) Total Magnesium amount for forest floor in total (g/m2) gramsPerSquareMeter n none
8 Potassium (g/m2) Oie Potassium amount for Oie (g/m2) gramsPerSquareMeter n none
9 Potassium (g/m2) Oa Potassium amount for Oa (g/m2) gramsPerSquareMeter n none
10 Potassium (g/m2) Total Potassium amount for forest floor in total (g/m2) gramsPerSquareMeter n none
11 Phosphorous (g/m2) Oie Phosphorous amount for Oie (g/m2) gramsPerSquareMeter n none
12 Phosphorous (g/m2) Oa Phosphorous amount for Oa (g/m2) gramsPerSquareMeter n none
13 Phosphorous (g/m2) Total Phosphorous amount for forest floor in total (g/m2) gramsPerSquareMeter n none
14 Manganese (g/m2) Oie Manganese amount for Oie (g/m2) gramsPerSquareMeter n none
15 Manganese (g/m2) Oa Manganese amount for Oa (g/m2) gramsPerSquareMeter n none
16 Manganese (g/m2) Total Manganese amount for forest floor in total (g/m2) gramsPerSquareMeter n none
17 Copper (g/m2) Oie Copper amount for Oie (g/m2) gramsPerSquareMeter n none
18 Copper (g/m2) Oa Copper amount for Oa (g/m2) gramsPerSquareMeter n none
19 Copper (g/m2) Total Copper amount for forest floor in total (g/m2) gramsPerSquareMeter n none
20 Zinc (g/m2) Oie Zinc amount for Oie (g/m2) gramsPerSquareMeter n none
21 Zinc (g/m2) Oa Zinc amount for Oa (g/m2) gramsPerSquareMeter n none
22 Zinc (g/m2) Total Zinc amount for forest floor in total (g/m2) gramsPerSquareMeter n none
23 Lead (g/m2) Oie Lead amount for Oie (g/m2) gramsPerSquareMeter n none
24 Lead (g/m2) Oa Lead amount for Oa (g/m2) gramsPerSquareMeter n none
25 Lead (g/m2) Total Lead amount for forest floor in total (g/m2) gramsPerSquareMeter n none

CODES

Variable: Plot
Description:
A counting number representing an existent plot

Data file: w100ffc.txt
Description: Hubbard Brook Forest Floor Data - Watershed 1 2000 - 100 15x15 cm excavated blocks Element concentrations
Column Variable Description Units Coded? Missing value label
1 Plot Plot# none y none
2 Calcium (ppm) Oie Calcium concentration for Oie (ppm of oven dry sample) number n none
3 Calcium (ppm) Oa Calcium concentration for Oa (ppm of oven dry sample) number n -8888
4 Magnesium (ppm) Oie Magnesium concentration for Oie (ppm of oven dry sample) number n none
5 Magnesium (ppm) Oa Magnesium concentration for Oa (ppm of oven dry sample) number n -8888
6 Potassium (ppm) Oie Potassium concentration for Oie (ppm of oven dry sample) number n none
7 Potassium (ppm) Oa Potassium concentration for Oa (ppm of oven dry sample) number n -8888
8 Phosphorous (ppm) Oie Phosphorous concentration for Oie (ppm of oven dry sample) number n none
9 Phosphorous (ppm) Oa Phosphorous concentration for Oa (ppm of oven dry sample) number n -8888
10 Manganese (ppm) Oie Manganese concentration for Oie (ppm of oven dry sample) number n none
11 Manganese (ppm) Oa Manganese concentration for Oa (ppm of oven dry sample) number n -8888
12 Copper (ppm) Oie Copper concentration for Oie (ppm of oven dry sample) number n none
13 Copper (ppm) Oa Copper concentration for Oa (ppm of oven dry sample) number n -8888
14 Zinc (ppm) Oie Zinc concentration for Oie (ppm of oven dry sample) number n none
15 Zinc (ppm) Oa Zinc concentration for Oa (ppm of oven dry sample) number n -8888
16 Lead (ppm) Oie Lead concentration for Oie (ppm of oven dry sample) number n none
17 Lead (ppm) Oa Lead concentration for Oa (ppm of oven dry sample) number n -8888

CODES

Variable: Plot
Description:
A counting number representing an existent plot

Data file: w100ffe.txt
Description: Hubbard Brook Forest Floor Data - Watershed 1 2000 - 100 15x15 cm excavated blocks Field data
Column Variable Description Units Coded? Missing value label
1 Plot Plot# none y none
2 E Code E horizon development intensity none y -9999
3 Major species (1) One of the three most important species directly over the plot none y XXXX
4 Major species (2) One of the three most important species directly over the plot none y XXXX
5 Major species (3) One of the three most important species directly over the plot none y XXXX
6 Total thickness (cm) (1) One of eight measures of thickness of forest floor block (in centimeters) centimeter n none
7 Total thickness (cm) (2) One of eight measures of thickness of forest floor block (in centimeters) centimeter n none
8 Total thickness (cm) (3) One of eight measures of thickness of forest floor block (in centimeters) centimeter n none
9 Total thickness (cm) (4) One of eight measures of thickness of forest floor block (in centimeters) centimeter n none
10 Total thickness (cm) (5) One of eight measures of thickness of forest floor block (in centimeters) centimeter n -9999
11 Total thickness (cm) (6) One of eight measures of thickness of forest floor block (in centimeters) centimeter n -9999
12 Total thickness (cm) (7) One of eight measures of thickness of forest floor block (in centimeters) centimeter n -9999
13 Total thickness (cm) (8) One of eight measures of thickness of forest floor block (in centimeters) centimeter n -9999
14 Oie thickness (cm) (1) One of eight measures of thickness of Oie (in centimeters) centimeter n none
15 Oie thickness (cm) (2) One of eight measures of thickness of Oie (in centimeters) centimeter n none
16 Oie thickness (cm) (3) One of eight measures of thickness of Oie (in centimeters) centimeter n none
17 Oie thickness (cm) (4) One of eight measures of thickness of Oie (in centimeters) centimeter n none
18 Oie thickness (cm) (5) One of eight measures of thickness of Oie (in centimeters) centimeter n -9999
19 Oie thickness (cm) (6) One of eight measures of thickness of Oie (in centimeters) centimeter n -9999
20 Oie thickness (cm) (7) One of eight measures of thickness of Oie (in centimeters) centimeter n -9999
21 Oie thickness (cm) (8) One of eight measures of thickness of Oie (in centimeters) centimeter n -9999

CODES

Variable: Plot
Description:
plots 1 to 200 from the top of the watershed to the base (not all plots were sampled)
Variable: E Code
Code
Description
0
none
1
weak -- "salt and pepper"
1.5
moderate
2
good -- clearly present
3
excellent -- deep and well-developed
Variable: Major species (1 - 3)
Code
Description
ACSA
Acer saccharum (Sugar Maple)
FAGR
Fagus grandifolia (American beech)
BEAL
Betula alleghaniensis (Yellow birch)
FRAM
Fraxinus americana (White ash)
ACSP
Acer spicatum (Mountain maple)
ACPE
Acer pensylvanicum (Striped maple or moose wood)
ABBA
Abies balsamea (Balsam fir)
PIRU
Picea rubens (Red spruce)
BEPA
Betula papyrifera (White or paper birch)
SOAM
Sorbus americana (Mountain ash)
ACRU
Acer rubrum (Red maple)

Data file: w100ffm.txt
Description: Hubbard Brook Forest Floor Data - Watershed 1 2000 - 100 15x15 cm excavated blocks Mass data
Column Variable Description Units Coded? Missing value label
1 Plot Plot# none y none
2 Oie Total kg/m2 Oven dry wt of total mass of Oie (kg/m2) kilogramsPerSquareMeter n none
3 OM Mass kg/m2 Oven dry wt of organic matter mass of Oie (kg/m2) kilogramsPerSquareMeter n none
4 LOI % Loss on Ignition of Oie (%) dimensionless n none
5 Oa Total kg/m2 Oven dry wt of total mass of Oa (kg/m2) kilogramsPerSquareMeter n none
6 OM Mass kg/m2 Oven dry wt of organic matter mass of Oa (kg/m2) kilogramsPerSquareMeter n none
7 LOI % Loss on Ignition of Oa (%) dimensionless n -8888

CODES

Variable: Plot
Description:
A counting number representing an existent plot